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. 2012 Oct 19;7(10):e48097. doi: 10.1371/journal.pone.0048097

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The fluorescence analysis was performed to compare the presence of peroxisomes (red) between (A, B) AfDsRED and (C, D) AfP33 strains. Cells not expressing p33 displayed a punctate pattern of fluorescence (A and detail in the boxed area) corresponding to scattered peroxisomes whereas cells expressing p33 showed the presence of red spots revealing an increased number of peroxisomes (C and detail in the boxed area). Nuclei are stained with DAPI. Scale bars = 10 mm (A–D); 5 mm (boxed areas in A and C).