Figure 1. Pentamidine inhibits LMP-1 TMD-5 ToxR activity.

(a), Dose-dependent curve of pentamidine inhibiting TMD-5 oligomerization measured by the ToxR assay (IC₅₀ = 9.1±0.6 µM). The effect of pentamidine on the E. coli. FHK 12 cells proliferation was examined by measuring the absorbance at 600 nm. The Miller unit and the absorbance at 600 nm of the untreated control was set as 100%. (b), ToxR measurement of the effect of pentamidine on the oligomerization of LMP-1 TMD-5 and TMD of diacylglycerol kinase (DAGK). Oligomerization activity in the absence of pentamidine was set as 100%. **p<0.01 by student t-test versus the control. (c), Western blotting showed the chimeric MBP-TMD-ToxR protein expression levels in E. coli. FHK 12 cells treated as (b). Endogenous MBP was served as the internal loading control.