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. 2012 Oct 19;7(10):e47703. doi: 10.1371/journal.pone.0047703

Figure 3. Coumarin fluorescence dequenching assay.

Figure 3

(a), Schematic representation of coumarin fluorescence dequenching assay. Coumarin labeled TMD-5 peptide forms trimers in the micelle and coumarin fluorescence is self-quenched. A small molecule that disrupts the TMD-5 oligomerization will suppress the quenching of coumarin fluorescence and a fluorescence increase will be observed. (b), Pentamidine disrupts LMP-1 TMD-5 trimers in the micelle. Pentamidine (64 µM) was added into 100 nM of coumarin labeled TMD-5 solution (50 mM HEPES, pH = 7.4) with 150 µM C14 betaine. Excitation, 360 nm; emission, 430 nm. *p<0.05 by student t-test versus DMSO control.