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. 2012 Oct 19;7(10):e47703. doi: 10.1371/journal.pone.0047703

Figure 4. 12% Bis-Tris SDS-PAGE analysis of TMD-5.

Figure 4

10 µL of TMD-5 peptide (KKKK-WQLLAFFLAFFLDLILLIIALYL-KKKK) and pentamidine mixture was incubated with equal volume of 2×Laemmli sample buffer at room temperature overnight. The final TMD-5 concentration was 200 µM. The samples were heated at 90°C for 7 min before loading. Electrophoresis was carried out at room temperature with NuPAGE MES SDS running buffer at 150 mV for 45 min. The resulting gel was stained by Coomassie blue staining. The ratios of compound to peptide tested were 0 (lane 2), 1 (lane 3), 4 (lane 4), 10 (lane 5) and 20 (lane 6).