Figure 5. Effect of TMD-5 trimer disruptor pentamidine on NF-κB activity (a, e), caspase3/7 activity (b, f), apoptosis (c, g) and cell proliferation (d, h) in EBV positive B 721 cells (a, b, c, d) and EBV negative B Ramos cells (e, f, g, h).

721 cells/Ramos cells were treated with the indicated concentration of pentamidine for 24 h. The cell proliferation rate was detected by WST-1 assay; constitutive LMP-1 NF-κB activity in 721 cells was determined by Steady-Glo luciferase assay system; NF-κB activity in Ramos cells were determined by SEAP assay; caspase 3/7 activity was detected by the Caspase-Glo® 3/7 assay system; cell apoptosis was detected by AO/EB method. It should be noted that the cell proliferation rate/NF-Κb activity of the untreated cells was normalized as 100% and the caspase 3/7 activity of the untreated control cells was set as 1. *p<0.05 by student t-test versus 0 µM pentamidine group; **p<0.01 by student t-test versus the 0 µM pentamidine group.