Figure 6. Attenuated proliferation of S. typhimurium in (A) spleen and (B) liver of Npc1^−/− mice.

Npc1 ^+/+, Npc1^+/− and Npc1 ^−/−, mice (age 6–8 weeks) were infected with S. typhimurium (1×10⁴ CFU) by i.p injection. At 48 hpi, mice were sacrificed, organs isolated and bacterial CFU were determined. The data obtained from 3 independent experiments are shown. n = 10 for Npc1 ^+/+ and Npc1 ^−/− and n = 8 for Npc1 ^+/−. Error bar show the mean±SEM. Student’s t test was carried out to determine the statistical significance.