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. 2012 Oct 19;7(10):e47480. doi: 10.1371/journal.pone.0047480

Figure 5. Effect of SELN on PTEN and GSK-3β phosphorylation.

Figure 5

(A) SOJ-6 and MiaPaCa-2 cells were starved then treated for 24h with SELN (16 nmoles cholesterol/ml) and lysed. Cell lysate proteins were separated on SDS-PAGE (80 µg of proteins/lane) and electrotransferred onto nitrocellulose membrane. The levels of PTEN, Ser380-phosphorylated PTEN (p-PTEN) GSK-3β, Ser9-phosphorylated GSK-3β (p-GSK-3β) and β-actin were determined by probing membranes with specific antibodies as indicated. Western blots are representative of three independent experiments. Lane 1, control performed in the absence of SELN, Lane 2, in the presence of SELN 3.0, Lane 3, in the presence of SELN 6.0. The right panels in figure 5A represents the quantification of western blots. (B) Ratios of Ser380-phosphorylated PTEN (p-PTEN) to total PTEN and Ser9-phosphorylated GSK-3β (p-GSK-3β) to total GSK-3β determined from A. Results are means (± SD) of three independent experiments using the NIH Image program (Mann-Whitney test).