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. 2012 Oct 19;7(10):e45686. doi: 10.1371/journal.pone.0045686

Figure 1. Viral IE86 protein interacts with pre-RC component Cdt1.

Figure 1

(A) 35S-methionine -labelled cellular pre-RC components Cdt1 and Orc2 as well as IE86 and gelsolin were expressed in rabbit reticulocyte lysates (inputs lanes 1–4) and tested for their ability to bind to GST (lanes 5–8), GST-IE86 (lanes 9–12) or GST-Geminin (lanes 13–16) as indicated. 1/10 input proteins are also shown (lanes 1–4) as well as GST inputs (lanes 17–19). (B) Alternatively, HFFF cells were co-infected with 1 pfu/cell AdCdt1 and 5 pfu/cell of HCMV or HCMV expressing IE86-EYFP for 48 h followed by immunoprecipitation with anti-Cdt1 antibody or a matched rabbit serum control and then western blotted with E13 (which detects both IE72 and IE86). Input proteins are also shown which clearly distinguish between IE72, IE86 and IE86-EYFP.