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. 2012 Oct 19;7(10):e45686. doi: 10.1371/journal.pone.0045686

Figure 2. Inhibition of DNA synthesis by IE86 does not correlate with an inability to load MCM3 or MCM7.

Figure 2

(A) Serum starved HFFF cells (−S) were treated with serum (+S) or treated with serum during infection with adenoviruses expressing GFP or IE86 (5 pfu/cell) as indicated for 48 h. After this time cells were co-stained for BrdU and IE protein or GFP as indicated. Quantitation of GFP or IE86 expressing cells which co-stained for BrdU incorporation using DAPI counterstaining (not shown) are indicated as averages +/−standard deviation from the mean from 5 independent fields of view. (B) Alternatively, serum starved HFFF cells (−S, lane 1) were treated with serum (+S, lane 2) or treated with serum during infection with AdGFP (Ad, lane 3) or AdIE86 (lane 4) as indicated and assessed for chromatin loading of Cdt1, MCM7 and MCM3. (C) MCM3 loading onto chromatin was assessed again in serum starved cells (−S) or serum stimulated cells (+S) in the presence of HCMV infection (HCMV). (D) Levels of IE86 expression and adenovirus hexon protein delivery in transduced cells were also analysed by western blot analysis.