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. 2012 Oct 19;7(10):e45686. doi: 10.1371/journal.pone.0045686

Figure 6. The inhibition of cellular MCM3AP expression prevents cell cycle arrest by viral IE86.

Figure 6

(A) HFFF cells were transfected with siRNAs to MCM3AP (siMCM3AP) or GANP (siGANP) or a control siRNA (siCon) for 24 h in the absence of serum and subsequently infected with AdIE86 (approximately 1 pfu/cell) in the presence of serum for 24 h as indicated. Cells were then fixed and co-stained for IE protein (green) and PCNA (red). (B) Serum starved HFFF cells (−S) were treated with serum (+S) and the percentage of PCNA positive cells enumerated after (Hoechst 33342) counterstaining. (C) HFFFs were infected with AdIE86 (1 pfu/cell) in the presence of serum and transfected with siRNAs to MCM3AP or GANP (siMCM3AP, siGANP) or with control siRNA (siCon) as indicated. Cells were then stained with anti-PCNA antibody and nuclei were counterstained with (Hoechst 33342) The percentage of IE expressing cells that were PCNA positive were quantified (results shown are averages with standard deviations from the mean of 5 independent fields of view).