TRE for the Free-Form automatic registration method (boxplots), the conventional manual registration method (gray region), and theoretical calculation [solid curve, Eq. 2] for various anatomical sites: (a) head, (b) thorax, and (c) abdomen. For all boxplots, the central mark represents the median TRE, the box represents the 25th and 75th percentiles, and the whiskers cover the range in TRE, excluding outliers (marked by a cross). The asterisk in (b) marks an outlier outside the plot.