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. 2012 Nov;40(11):2119–2125. doi: 10.1124/dmd.112.046466

Fig. 3.

Fig. 3.

Depleting CYP2S1 expression in human lung cells promotes cell proliferation in BEAS-2B cells. A, alamarBlue proliferation assay was used to monitor cell proliferation. Proliferation was significantly increased in CYP2S1-depleted BEAS-2B cells (759 and 984, crosshatched and horizontal line bars, respectively) compared with SCRAM (closed bars). Data represent the mean ± S.D. of alamarBlue reduction at 24 and 48 h. One-way analysis of variance was used to determine differences between groups followed by post hoc t tests. B, the MTT was also assessed to evaluate differences in cell proliferation after 24 h of growth. Cell viability was also found to be significantly increased in CYP2S1-depleted BEAS-2B cells (759 and 984, crosshatched and horizontal line bars, respectively) compared with SCRAM (closed bars). Statistical analysis was performed using the Student's t test. *, a significance of p < 0.05; **, a significance of p < 0.01; and ***, a significance of p < 0.001.