Fig. 5.
Induction of apoptosis of Jurkat cells after treatment with CTA056. Jurkat cells were seeded at 106 cells/ml (2 ml) in a six-well plate and treated with CTA056 at the indicated concentrations for 24 h. Cell-cycle arrest was analyzed using PI staining (A). Apoptosis was analyzed using Annexin-V-FITC apoptosis detection kit (Abcam, Inc.) (B). Columns, mean; bars, S.D., n = 3. 125, 250, 500, and 1000 nM are significantly different from control (*, p < 0.05, one-way ANOVA with Tukey's test for pairwise comparison).