Fig. 3.

Effect of DAX-1 mutations on CAR variant activity. Results shown here represent single transfection experiments, with all of the treatments in quadruplicate. COS-1 cells were transfected with the 3.1-RXRα expression vector, the 2B6-XREM-PBREM reporter, the pRL-CMV vector for normalization of transfection efficiency, and varying amounts of CMV2-CAR1, -CAR2, or -CAR3 and PCMV6-empty, -DAX1 WT, -DAX1 M1, -DAX1 M2, -DAX1 M3, or -DAX1 PCF (A) or PCMV6-DAX1-TSD (B). All of the treatments were for 24 h, data are represented as normalized luciferase values, and each data point represents the mean (±S.D.). Asterisks indicate significant difference from respective control (no DAX1) within a chemical treatment group (p < 0.05).