TABLE 1.
Primers used for creation of DAX-1 constructs
| Construct | Primer | Sequence |
|---|---|---|
| VP16-DAX1 | Forward (BamHI) | 5′-ATGCGGATCCTTGCGGGCGAGAACCACCAGTG-3′ |
| Reverse (XbaI) | 5′-ATGCTCTAGATTATATCTTTGTACAGAGCATTTCC-3′ | |
| pCMV6AC-DAX1-M1 | Sense | 5′-GGGCAGCATCCTCTACAACGCGGCTATGAGCGCGAAGCAAACG-3′ |
| Antisense | 5′-CGTTTGCTTCGCGCTCATAGCCGCGTTGTAGAGGATGCTGCCC-3' | |
| pCMV6AC-DAX1-M2 | Sense | 5′-GGCAGCATCCTCTACAGCGCGGCGACGAGCGCAAAGCAAAC-3′ |
| Antisense | 5′-GTTTGCTTTGCGCTCGTCGCCGCGCTGTAGAGGATGCTGCC-3′ | |
| pCMV6AC-DAX1-M3 | Sense | 5′-GGGCAGCATCCTCTACAGCGCGGCCACTAGCTCAAAGCAAACG-3′ |
| Antisense | 5′-CGTTTGCTTTGAGCTAGTGGCCGCGCTGTAGAGGATGCTGCCC-3′ | |
| pCMV6AC-DAX1-PCF | Sense | 5′-GCCCTGCTTCCAGGCGGCGCCCCTGGACCAGC-3′ |
| Antisense | 5′-GCTGGTCCAGGGGCGCCGCCTGGAAGCAGGGC-3′ | |
| pCMV6AC-DAX1-TSD | Forward (BamHI) | 5′-ATGCGGATCCTTGCGGGCGAGAACCACCAGTG-3′ |
| Reverse (XhoI) | 5′-GTCTCGAGTTATTGGCATTGATGAATCTCAGCAGG-3′ |