Fig. 2.

EGCG (10 nM) increases cardiomyocyte Ca²⁺ transients and contractility. A, examples of traces of Ca²⁺ fluorescence recordings from field-stimulated (1 Hz) cardiomyocytes after a 5-min exposure to EGCG (10 nM) or vehicle (water). Rapid caffeine application (10 mM) was used to estimate SR Ca²⁺ content. Fractional Ca²⁺ release was calculated as the ratio between the amplitude of the field-stimulated Ca²⁺ transient and caffeine-induced Ca²⁺ transient. B and C, comparison of average cardiomyocyte shortening (B) and fractional Ca²⁺ release (C). Data are from three independent myocyte preparations. n = 25/group, *, p < 0.05 versus vehicle.