Fig. 7.

EGCG sensitizes RyR2 channels to activation by cytosolic Ca²⁺. A, concentration-effect curve of EGCG on specific binding of [³H]ryanodine to cardiac SR membranes. B, EGCG (10 μM) significantly increases the sensitivity of [³H]ryanodine binding to Ca²⁺ in the assay buffer (EC₅₀ = 6.2 ± 2.2 and 1.8 ± 0.1 for vehicle control and EGCG, respectively; p < 0.01). Data are means± S.D. of n = 3 determinations, each performed in duplicate.