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. 2012 Nov;82(5):1001–1007. doi: 10.1124/mol.112.079863

Fig. 3.

Fig. 3.

Role of RIP1 in APAP-induced hepatotoxicity. A, primary hepatocytes from WT and KO mice were treated with 5 mM APAP for 6 h, in the presence or absence of 30 μM necrostatin-1 (Nec-1). Expression and phosphorylation of JNK and expression of RIP1 were examined through immunoblot analyses. RIP1 activity was determined through in vitro kinase assays with ATP and myelin basic protein (MBP) as substrates. Representative data from two independent experiments are shown. B, necrosis was determined through Sytox green staining after 20 h of treatment of WT and KO hepatocytes with increasing amounts of APAP, in the presence or absence of 30 μM necrostatin-1. Results are mean ± S.D. from two independent experiments. *, p < 0.01; **, p < 0.001. NS, not significant.