Fig. 3.

Specificity of NSC13746 and P6981 in the inhibition of the DNA binding of unique B-ZIP domains. The dimer (15 nM) of C/EBPα (A), CREB (B), or C/EBPβ (C) B-ZIP protein was incubated with increasing concentrations of P6981 or NSC13746 (18, 32, 56, and 100 nM for C/EBPβ; 5.6, 10, 18, 32, 56, and 100 nM for CREB and C/EBPα) and assayed by EMSA. IC₅₀ values were estimated and shown in Supplemental Table 1.