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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: ACS Chem Biol. 2012 Aug 10;7(10):1636–1640. doi: 10.1021/cb300171p

Figure 2.

Figure 2

Circular permutation of mito-ZifCY1 dramatically increases its dynamic range. a) The N-and C-termini of cpV FPs are relocated to 5 different loops of the original Venus FP at the amino acid positions 49, 157, 173, 195, and 229. b) The average R of the unbound and bound sensors expressed in at least 10 cells from 2 or more independent experiments, acquired using identical exposure times, are summarized. c) High dynamic range sensors report lower fractional saturation.