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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Nat Immunol. 2012 Oct 7;13(11):1110–1117. doi: 10.1038/ni.2427

Figure 5. USP25 and its DUB activity are required for restriction of IL-17 signaling.

Figure 5

(a) Immunoblot of IκBα, phosphorylated IκBα (p-IκBα), and phosphorylated MAPKs in lysates from wild-type and Usp25-/- MEFs stimulated with IL-17 (100 ng/ml) or TNF (10 ng/ml) for the indicated time points. (b) Real-time analysis of Cxcl1 and Il6 mRNA in wild-type and Usp25-/- MEFs reconstituted with empty vector (vector), USP25 or USP25(C178S) left untreated (-) or treated with IL-17 (50 ng/ml) or TNF (10 ng/ml) or IL-17 plus TNF. (c) Immunoblot of IκBα, phosphorylated IκBα (p-IκBα), and phosphorylated MAPKs in lysates from wild-type and Usp25-/- MEFs reconstituted with empty vector (vector), USP25 or USP25(C178S) treated with IL-17 (50 ng/ml) for the indicated time points. The intensity of p-IIκBα and IIκBα bands was quantified with the ImageJ program and the ratios of p-IIκBα/IIκBα were shown in the graph. Data are representative of three independent experiments. For b, graphs show mean ± SD, n = 3. *p<0.05; **p<0.01.