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Labeling of adenylating enzymes in crude cell lysates. A. Labeling of overexpressed MbtA in E. coli cell lysate. B. Labeling of overexpressed EntE in E. coli cell lysate. C. Mass spec detection of labeled MbtA from M. smegmatis. C. LC-MS/MS analysis of labeling of M.smegmatis MbtA by probe 6 and competition with Sal-AMS.
