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Hfq binding to Qrr1 sRNA. Representative EMSAs for VcHfq, VcHfq72, VcNTDEcCTR, EcHfq, EcHfq72, and EcNTDVcCTR binding to Qrr1 sRNA. 32P-labelled Qrr1 was at 5 nM and the concentrations of hexameric protein are specified above the lanes. The mobilities of free Qrr1 RNA, the 1:1 Qrr1:Hfq complex, and higher-order complexes are indicated on the right.
