Skip to main content
. 2012 Jan 27;36(1-2):32–42. doi: 10.1016/j.immuni.2011.11.015

Figure 1.

Figure 1

PKCδ Is Tyrosine Phosphorylated upon Dectin-1 Ligation

(A) BMDCs were left untreated (Ø) or stimulated for 10 min with zymosan (Z). Cellular lysates were immunoblotted with phospho-Tyrosine (p-Tyr) antibodies.

(B) BMDCs were stimulated with zymosan as in (A). Proteins were immunopurified with p-Tyr antibodies or IgG1 control (isotype) and visualized by Coomassie staining after electrophoresis.

(C) BMDCs were left untreated or stimulated with zymosan as in (A). Proteins from lysates were immunoprecipitated with PKCδ antibodies or isotype control and analyzed by immunoblot with p-Tyr or PKCδ antibodies.

(D) Immunoblot analysis of BMDCs stimulated with zymosan for various times and probed with antibodies against phospho-PKCδ (Tyr311) or β-actin.

(E) BMDCs were left untreated or preincubated with Syk inhibitor R406 (0.5, 1, 2 μM), Src-kinase inhibitor PP2 (1.5, 3, 6 μM), or its inactive analog PP3 (1.5, 3, 6 μM) and stimulated for 10 min with zymosan. Lysates were analyzed by immunoblot with antibodies against phospho-PKCδ, PKCδ, phospho-PLCγ2, or PLCγ2.

All results are representative of at least three independent experiments.