Fig. 1.

Microscopic images of C2C12 culture and differentiation steps. Fibroblast like C2C12 myoblasts (1) were grown in DMEM + 20 % FBS and kept in 37 °C with 5 % CO₂ until reaching about 80 % confluence (2). Then culture medium replenished with DMEM + 2 % horse serum for inducing myoblast differentiation to myocytes (3&4) and polynuclear myotubes (5&6). Cells on day 6 were prepare for adding Hydro-Alcoholic Cinnamon Extract (HACE) or DMSO as intervention or vehicle categories respectively