In situ hybridization with probes against Otx1 (A-E), Hmx3 (F-J), Lmo4 (K-O) and Gata3 (P-Y) shows unchanged expression in E10.5 Chd7+/+ (A, F, K, P, U), Chd7Gt/+ (B, G, L, Q, V) and Foxg1-Cre;Chd7+/flox (D, I, N, S, X). Otx1 (E) and dorsal Gata3 (T) expression are reduced in CKO embryos, whereas Hmx3 (J), Lmo4 (O), and ventral Gata3 (Y) expression are preserved. Hypoplastic Chd7Gt/Gt otocysts show no Otx1, Hmx3, Lmo4 or dorsal Gata3 expression, but maintain ventral Gata3 expression (W). All sections are in the transverse orientation with dorsal towards the top as shown by the arrows in (A). Bold lines delimit expression domains. Abbreviation: CKO = Chd7 conditional knockout (Foxg1-Cre;Chd7Gt/flox).