In situ hybridization with probes against Lmx1a (A-D), Gbx2 (E-H), Otx1 (I-L), Dlx5 (M-P) and Hmx3 (Q-T) show unaltered expression patterns in E11.5 Chd7 +/flox (A, E, I, M, Q), conditional heterozygous (B, F, J, N, R) and Chd7Gt/+ (C, G, K, O, S) ears. Lmx1a (D), Gbx2 (H), Hmx3 (T) and medial Dlx5 (P) expression were preserved in Chd7 CKO (Foxg1-Cre;Chd7Gt/flox) ears. Lateral Dlx5 (P) and Otx1 (L) expression were absent from CKO inner ears. All sections are in the transverse orientation at the level of the lateral semicircular canal. Bold lines delimit expression domains. Abbreviation: CKO= Chd7 conditional knockout (Foxg1-Cre;Chd7Gt/flox).