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Existence of SP cells in the established hepatoma cell lines. Hepatoma cell lines were plated one day before analysis to achieve approximately 30% confluence on the day of analysis. The cells were detached, labeled with the Hoechst 33342 in the presence or absence of 50 μM verapamil and then analyzed by flow cytometry. The SP cells which disappeared in the presence of verapamil (lower panel) are outlined and shown as a percentage of the total cell population.
