Figure 4.

THL inhibited the proliferation and colony formation of Huh7 SP cells. (a) Proliferation inhibition effects of different doses of THL on Huh7 SP cells. (b) Colony formation inhibition effects of THL on Huh7 SP cells. Huh7 SP cells were seeded into 6-well plates in DMEM/F12 medium (supplement with B27 supplement, bFGF and EGF). After 24 h, the cells were treated with the indicated concentrations of THL for 14 days. At harvest, the SP cells were fixed and stained with crystal violet to visualize the colonies for counting. *P < 0.05; **P < 0.01, indicating statistical significance as compared to the untreated control SP cells.