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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Curr Protoc Mol Biol. 2012 Jul;CHAPTER:Unit10.29. doi: 10.1002/0471142727.mb1029s99

Figure 2.

Figure 2

Outline of Basic and Alternate Protocols 1. Both assays are used to measure E1-catalyzed ATP:PPi exchange rate but the detection method for the radioactive ATP produced in the reactions are different. Steps 1–6 are the same for both protocols. In Basic Protocol 1, after the reaction is quenched, [32P]ATP is adsorbed by a charcoal slurry and detected using Cherenkov counter. In Alternate Protocol 1, the [32P]ATP is adsorbed in an activated charcoal paper and detected using a PhosphoImager.