Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Plots of E1-catalyzed ATP:PPi isotope exchange assay at different SUMO concentrations. The plots are generated based on the assays outlined in Basic and Alternate Protocols 1. (A) Plot of rate of ATP:PPi exchange (pmole/min) versus SUMO concentration (μm). (B) Double-reciprocal (Lineweaver-Burk) plot of (A) to linearized the data and determine the kinetic constants (K1/2 and Vmax).
