Figure 6.

Strategy for identification of RvD1-GPCR circuits. Self-limited zymosan-stimulated mouse peritonitis was used to obtain resolving exudates that were collected 4, 12, 24, and 48 h after zymosan A injection for temporal and differential analysis of leukocyte counts and phenotype, miRNA real-time PCR analysis, and lipidomics as in Recchiuti et al. (2011) and Krishnamoorthy et al. (2012). Candidate miRNAs, defined as those that were temporally and/or RvD1-regulated, were confirmed using bioinformatics and prediction of target genes, real-time PCR, as well as overexpression in human MΦs. The Ingenuity Pathway Analysis Software database was used to identify circuits of molecules and biological functions controlled by RvD1-regulated miRNAs and target genes (see text; Recchiuti et al., 2011 for analysis details).