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. Author manuscript; available in PMC: 2012 Oct 22.
Published in final edited form as: Science. 2012 May 4;336(6081):593–597. doi: 10.1126/science.1218498

Fig. 3.

Fig. 3

Lig3- and PARP1-dependent alt-NHEJ in the absence of shelterin. (A) Metaphase chromosomes of the indicated MEFs analyzed (as in Fig. 2D) at 108 hours after Cre. (B) Quantification of telomere fusions in the indicated MEFs at 108 hours after H&R-Cre. Bars and not error bars means of three independent experiments, ± SDs. (C) Quantification of telomere fusions induced by deleting TRF1 and TRF2 [as in (A)] after treatment with PARP1 shRNA or 0.5 μM olaparib. (D) Quantification of telomere fusions [as in (C)] in cells treated with Lig3 or control shRNA. (E) Alt-NHEJ in G0 arrested TRF1F/FTRF2F/FKu80−/−p53+/+Cre-ERT2 MEFs. MboI and AluI digested DNA resolved on a pulsed-field gel electrophoresis probed with end-labeled [AACCCT]4. Dashed and solid lines: fused and unfused telomeres, respectively. Day 4R: cells released on day 4 and analyzed on day 5. (F) Percentage of fused telomeres in Ku-deficient MEFs lacking the indicated shelterin subunit(s). Cells were analyzed at 108 hours after Cre-mediated deletion of the floxed alleles of shelterin. (G) Summary of the repression of Lig3- and PARP1-dependent alt-NHEJ by shelterin and Ku70/80.