Fig 3.

DLX4 promotes migratory and invasive activity in cancer cell lines. A. Wound-healing assay. HEK293-DLX4 cells were plated in a six-well dish. Then linear wounds were created by scraping confluent cell monolayers after the cells were treated with DOX or vehicle for 12 h. The “wounded” areas were examined at the indicated time points under 100× magnification. B. Cell migration assay. **, p<0.001. C. Cell invasion assay. **, p<0.001. D. Cell proliferation assay. Migration and invasion assays were performed using HEK293-DLX4 and the empty vector control cells with or without DOX induction using transwell plates described in the Materials & Methods section. Cell proliferation was assayed using a CCK-8 kit. D&E. Cell migration and invasion assays in HeLa cell over-expressing of pcDNA3-DLX4 or pcDNA3 empty vector. **, p<0.001. G. Representative migration assay image at Fig. 3D.