Fig 5.

Expression of DLX4 and TWIST in human breast tumor tissues. A. DLX4 and TWIST expression in human breast tumor tissues. Tumor tissues from 28 patients with invasive ductal breast tumors were collected, whole tissue extracts were prepared and western blotting analysis was performed with the indicated antibodies. Note that 24 of 28 samples were DLX4 positive (85.7%). B. Representative immunohistochemical analysis of TWIST and DLX4 proteins in invasive ductal breast tumors. TWIST protein expression was positively correlated with DLX4 expression in most of the breast tumors. Note that I and III were TWIST and DLX4 expression positive in one tissue, whereas II and IV were TWIST and DLX4 negative respectively in others.