Figure 3.

Ionic strength affects the surface hydrophobicity of 14-3-3 proteins. Emission spectra of bis-ANS, a fluorescent probe, to monitor changes in GF14ω surface hydrophobicity. A final concentration of 5 μm GF14ω in 100 mm Mops, pH 7.5, 10 mm Mg²⁺, with the addition of 1 μm bis-ANS, was used as the control. To this mixture 50 or 100 mm KCl was added, and the bis-ANS emission spectrum was recorded. The excitation wavelength was 385 nm, and the emission spectra were recorded from 400 to 600 nm.