Table II.

Alignment of sites in various proteins involved in the interaction with 14-3-3 proteins

Protein	Phospho-Ser	Residue Position
			−2		0		+2		+4		+6		+8		+10
Raf-1	Ser-259	R	S	T	S	T	P	N	V	H	M	V	S	T	T	L
	Ser-621	R	S	A	S	E	P	S	L	H	R	A	A	H	T	E
β-Raf-1	Ser-364	R	S	S	S	A	P	N	V	H	I	N	T	I	E	P
	Ser-728	R	S	A	S	E	P	S	L	N	R	A	G	F	Q	T
PKCβ	Ser-241	R	R	L	S	V	E	I	W	D	W	D	L	T	S	R
Bcr	Ser-95	A	S	A	S	R	P	Q	P	A	P	A	D	G	A	D
	Ser-371	R	S	P	S	Q	N	S	Q	Q	S	F	D	S	S	P
cdc25a	Ser-106	R	I	H	S	L	P	Q	K	L	L	G	C	S	P	A
	Ser-191	R	D	S	S	E	P	G	N	F	I	P	L	F	T	P
cdc25b	Ser-216	R	P	S	S	A	P	D	L	M	C	L	S	P	D	R
Tyr hyd	Ser-349	R	H	A	S	S	P	M	H	S	P	E	P	D	C	C
Trp hyd	Ser-260	R	H	S	S	D	P	F	Y	T	P	E	P	D	T	C
NR	Ser-543	R	T	A	S	T	P	F	M	N	T	T	S	K	M	Y

All amino acids are coded using the standard single-letter codes. The abbreviated protein names are: Raf-1 kinase, Raf-1; β Raf-1 kinase, β-Raf-1; protein kinase C β, PKCβ; Tyr hydroxylase, Tyr hyd; and Trp hydroxylase, Trp hyd. The numbering of a residue position is relative to the phosphorylatable Ser at position 0. Ser and Thr residues that are present C terminal to the phosphorylatable Ser are shown in boldface, underlined type. Note that with one exception, all target proteins contain at least one Ser/Thr residue C terminal to the recognized binding motif (for review, see Aitken, 1996).