Table III.

Metabolites and physical activators reduce the association of 14-3-3 proteins with pNR

Addition	Inhibition of NRA					Inhibition by 14-3-3
	−GF14ω	+GF14ω	GF14ω	−14-3-3	+14-3-3
	% of control					%
pH 7.5, +Mg2+
None	100	54	46	100	73	27
5 mm 5′-AMP	126	107	15	134	125	8
5 mm 3′-AMP	109	67	39	115	92	20
5 mm 2′-AMP	105	61	42	111	88	21
5 mm 5′-AMS	101	60	41	93	71	24
10 mm KCl	98	59	40	103	79	8
50 mm KCl	111	83	25	117	99	15
100 mm KCl	118	103	13	121	114	6
10 mm Pi	109	77	30	106	85	20
pH 6.5, −metal ion
None	100	69	31	100	79	21
5 mm 5′-AMP	120	102	15	123	112	9
5 mm 3′-AMP	109	82	25	105	85	19
5 mm 2′-AMP	106	76	28	108	89	18
5 mm 5′-AMS	102	74	27	107	87	19
10 mm KCl	104	78	25	101	85	16
50 mm KCl	107	85	21	105	96	9
100 mm KCl	127	113	11	131	127	3
10 mm Pi	115	102	11	109	91	17

pNR was incubated with and without endogenous spinach 14-3-3 proteins (mixture of isoforms, 20 μm) or recombinant GF14ω (5 μm), with additions as indicated, on ice for 5 min. NRA was then determined in the same conditions as the preincubation. Results are expressed as a percentage of the control activity and all values are means of two determinations.