Figure 3. Structural analysis of RVFW specimen.

A, RVFW specimen after decellularization. B, collagen fibre architecture of decellularized specimen as measured by SALS. The vector field represents the local dominant collagen fibre orientations, which shows a strong alignment with the apex-to-outflow tract direction. The normalized orientation index (NOI) codes the degree of local fibre alignment, which is very high in the region of mechanical testing, indicating that the local fibre orientation is almost uniformly oriented along the X₁ direction. C, transmural myofibre and collagen fibre orientations showing very little variation around 0 deg line (corresponding to the apex-to-outflow-tract direction).