Figure 1.

Relative increase in the steady-state level of CYP76B1, CYP81B1, and CYP73A1 transcripts induced by xenobiotics. RNA was prepared from Jerusalem artichoke tuber tissues, sliced, and aged in water or in solutions of different chemicals. The same RNA-blot membrane (20 μg of total RNA in each lane) was successively hybridized with CYP81B1, CYP73A1, and CYP76B1 ³²P-radiolabeled probes. Hybridization signals were quantified with a phosphor imager. Induction is calculated by comparison with the signal recorded with RNA from tissues aged in water. Induction obtained with Flav, NA, and B(a)P, which were dissolved in DMSO, was compared with that obtained for tissues treated with DMSO alone.