Figure 1.

Experimental Protocol. A) Representative time plot of the experimental protocol. B) Summary data (mean±sem) of maximum Ca²⁺ transient amplitude with 1 mM [Ca²⁺]_o, 3mM [Ca²⁺]_o, or ISO from WT (clear bar) and NOS1^−/− (black bar) myocytes. C) Summary data (mean±sem) of Ca²⁺ transient decline measured as the RT₅₀. a P<0.05 vs corresponding WT, b P<0.05 vs 1 mM [Ca²⁺]_o, c P<0.05 vs 1mM and 3mM [Ca²⁺]_o.