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. Author manuscript; available in PMC: 2012 Oct 22.
Published in final edited form as: Biochem J. 2011 Jan 1;433(1):245–252. doi: 10.1042/BJ20101293

Figure 1. XBP1s is a target of acetylation.

Figure 1

(A) HEK293 cells were transfected with Flag-XBP1s and increasing amount of p300. Cell lysates were analyzed by immunoprecipitation and Western blotting. Ace-K, acetylated lysine. *, non-specific band. (B) Equal amount of XBP1s was cotransfected with p300 or a control vector into HEK293 cells. Cells were harvested for lysates after cycloheximide (100 μg/ml) treatment for indicated time period. Cell lysates were analyzed by Western blotting.