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. Author manuscript; available in PMC: 2012 Oct 22.
Published in final edited form as: J Immunol. 2010 Jul 14;185(4):2009–2012. doi: 10.4049/jimmunol.1000855

Figure 2. KIR Antisense Transcripts Negatively Regulate KIR Gene Expression.

Figure 2

KIR3DL1 antisense and sense transcripts were separately cloned into the MSCV-eGFP vector. An additional KIR3DL1 antisense MSCV-eGFP construct lacking the 28 base segment was also generated. Retroviral supernatants were used to transduce CD34+ cells, which were developed into NK cells. (A) FACS plots representative of 4 experiments showing the surface KIR expression for cells transduced with each construct. (B) qRT-PCR comparison of KIR gene expression in cells transduced with either the KIR3DL1 Antisense vector or the control eGFP vector. Values are presented as a fold ratio with 1 equal to KIR expression observed with eGFP-only vector. Percent homology with the KIR3DL1 promoter is indicated below the graph. Data was pooled from 4 independent experiments, and error bars represent the standard error values between experiments. P values comparing KIR transcript expression between isolated NK cell populations were derived using a Student’s t test.