Table 1.

Initial suspension composition (before spray-drying), theoretical composition of the spray-dried formulation, and ITZ experimental content as determined by HPLC

Suspension	ITZ (% w/v)		TPGS (% w/v)		TPGS (% w/wITZ)
Initial suspension compositions in water/isopropanol (2:1)
S1	0.33		0.017		5
S2	0.33		0.033		10
S3	0.5		0.025		5
S4	0.5		0.050		10
S5	1		0.050		5
S6	1		0.1		10
DPI theoretical composition after spray-drying					Experimental ITZ content
Formulation	ITZ (% w/w)	Mannitol (% w/w)	TPGS (% w/w)	NaTau (% w/w)	Mean ITZ content (% wt)	CV (%)	Relative error (%)
Ca	32.258	64.516	3.226	–	30.6	2.8	−5.2
N1b	90.91	–	9.09	–	90.2	2.4	−0.76
N2b	32.258	64.516	3.226	–	34.1	3.8	5.71
N3b,c	32.154	64.309	3.215	0.322	27.4	9.4	−14.9
N4b,c	31.949	63.898	3.195	0.958	24.1	2.02	−24.5

Notes:

^aProduced from the initial suspension S6;

^bproduced from the S6 nanosuspension;

^cNaTau added to the carrier solution before spray-drying.

Abbreviations: CV, coefficient of variation; DPI, dry powder for inhalation; HPLC, high-performance liquid chromatography; ITZ, itraconazole; NaTau, sodium taurocholate; TPGS, tocopherol polyethylene 1000 succinate.