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. 1982 Nov;38(2):774–777. doi: 10.1128/iai.38.2.774-777.1982

Propagation and primary isolation of papovavirus JC in epithelial cells derived from human urine.

A M Beckmann, K V Shah, B L Padgett
PMCID: PMC347804  PMID: 6292111

Abstract

Human papovavirus JC, previously passaged in amnion cells or in primary human fetal glial cells, replicated efficiently in urine-derived epithelial cells. Primary isolation of the virus from brain extracts was possible in urine-derived cells, but these cells were not as sensitive as primary human fetal glial cells for this purpose. Primary isolations of human papovavirus JC from urine sediments of renal transplant patients were made in urine-derived cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Felix J. S., Sun T. T., Littlefield J. W. Human epithelial cells cultured from urine: growth properties and keratin staining. In Vitro. 1980 Oct;16(10):866–874. doi: 10.1007/BF02619424. [DOI] [PubMed] [Google Scholar]
  2. Miyamura T., Yoshiike K., Takemoto K. K. Characterization of JC papovavirus adapted to growth in human embryonic kidney cells. J Virol. 1980 Aug;35(2):498–504. doi: 10.1128/jvi.35.2.498-504.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Osborn J. E., Robertson S. M., Padgett B. L., ZuRhein G. M., Walker D. L., Weisblum B. Comparison of JC and BK human papovaviruses with simian virus 40: restriction endonuclease digestion and gel electrophoresis of resultant fragments. J Virol. 1974 Mar;13(3):614–622. doi: 10.1128/jvi.13.3.614-622.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Padgett B. L., Rogers C. M., Walker D. L. JC virus, a human polyomavirus associated with progressive multifocal leukoencephalopathy: additional biological characteristics and antigenic relationships. Infect Immun. 1977 Feb;15(2):656–662. doi: 10.1128/iai.15.2.656-662.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Padgett B. L., Walker D. L. Prevalence of antibodies in human sera against JC virus, an isolate from a case of progressive multifocal leukoencephalopathy. J Infect Dis. 1973 Apr;127(4):467–470. doi: 10.1093/infdis/127.4.467. [DOI] [PubMed] [Google Scholar]
  6. Sack G. H., Felix J. S., Lanahan A. A. Plaque formation and purification of BK virus in cultured human urinary cells. J Gen Virol. 1980 Sep;50(1):185–189. doi: 10.1099/0022-1317-50-1-185. [DOI] [PubMed] [Google Scholar]
  7. Shah K. V., Ozer H. L., Ghazey H. N., Kelly T. J., Jr Common structural antigen of papovaviruses of the simian virus 40-polyoma subgroup. J Virol. 1977 Jan;21(1):179–186. doi: 10.1128/jvi.21.1.179-186.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Takemoto K. K., Howley P. M., Miyamura T. JC human papovavirus replication in human amnion cells. J Virol. 1979 Apr;30(1):384–389. doi: 10.1128/jvi.30.1.384-389.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wroblewska Z., Wellish M., Gilden D. Growth of JC virus in adult human brain cell cultures. Arch Virol. 1980;65(2):141–148. doi: 10.1007/BF01317325. [DOI] [PubMed] [Google Scholar]

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