Table 1.
Characterization of four nuclear microsatellites in six Silene ciliata populations
| Locus | Repeat motif | Primer sequences (5′–3′) | Ta (°C) | Size range (bp) | NA | GB |
|---|---|---|---|---|---|---|
| Sci-1224 | (CT)18 | F: NED-ACCTGATTAGAAGACACAGGAGGA | 56 | 140–196 | 26 | JF979125 |
| R: TTTATGTTGCCGCATCCTTATC | ||||||
| Sci-1208 | (CT)10 | F: PET-TGGAAACGATGTATGAGACGA | 56 | 158–182 | 11 | JF979126 |
| R: TGTGATTGAAGTAGCCAAACCT | ||||||
| Sci-0106 | (CT)7 | F: VIC-AAACAAACGAGCGATCATCTAA | 56 | 111–135 | 12 | JF979127 |
| R: TTCCGATGCTTCTGGTACTTCT | ||||||
| Sci-1443 | (CT)7 | F: FAM-GACGCCTTTCTCAAAATCACC | 56 | 126–160 | 11 | JF979128 |
| R: GGGAATCTAGGGTTTAGCAGTGA |
The repeat motif, forward (F) and reverse primer (R) sequence, annealing temperature (Ta), size range, total number of alleles (NA) and GenBank accession number (GB) are given for each locus.