Figure 7.

Gβ₃ deletion heavily affects modulators of the mGluR6 cascade that display a punctate appearance, but only mildly affects the diffusely distributed modulators. A, Immunostaining of WT (top) and Gnb3-null (KO; bottom) mice for Gβ₅, R9AP, and RGS11. Punctate staining is greatly reduced in the null mouse. B, Relative immunostaining (as defined in Fig. 6C) for these proteins in the outer plexiform and inner nuclear layers of the retina (white bars), relative puncta count (dotted bars), and band intensity from Western blots (for RGS11 only; dark bar). Punctate staining is greatly reduced in the null mouse. C, Immunostaining of WT (top) and Gnb3-null (KO; bottom) mice for the diffuse modulators PKC and Ret-PCP2. D, Western blot for RGS11 shows that the band intensity is similar between wild-type and Gnb3-null mice. E, Relative immunostaining of various layers of the retina (white bars) and sum intensities of all layers (gray bars). *p < 0.05; **p < 0.01. Abbreviations are defined as in Figure 1.