Table 2.
Flow cytometric analysis of 4T1 and MIN-O tumor cells. Tumor cell-suspensions were incubated with 2B11-PE. The fluorescent signal emitted by CXCR4 positive cells was measured (n=3 per tumor type). The mean fluorescent intensity ratios (MFIR) were determined. The percentage of lymphocytes within the CXCR4 positive population was determined after co-incubation with 2B11-PE and an Alexa-Fluor700 labeled anti-CD45 antibody.
| Tumor type | MFIR | %lymphocytes |
|---|---|---|
| MIN-O Early stage (<100mm3) | 2.25 ± 0.17 | 0.43 ± 0.002 |
|
| ||
| MIN-O Intermediate stage (100-400mm3) | 4.32 ± 0.02 | 2.07 ± 0.001 |
|
| ||
| MIN-O Late stage (>400mm3) | 5.36 ± 1.80 | 3.48 ± 0.004 |
|
| ||
| 4T1 Late stage (>400mm3) | 1.70 ± 1.07 | 3.39 ± 0.002 |