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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: J Immunol. 2012 Feb 6;188(6):2712–2721. doi: 10.4049/jimmunol.1100903

Figure 6. Inhibition of SIRT1 activity by Ex-527 promotes differentiation of iTregs.

Figure 6

(A) Intracellular flow cytometric analysis of Foxp3 expression in iTregs differentiated at indicated concentrations of TGF-β in the presence and the absence of Ex-527 (50 μM). One experiment performed in triplicate is shown. (B) Intracellular flow cytometric analysis of Foxp3+ cells and MFI of intracellular Foxp3 in iTregs after treatment with Ex-527 (50 μM) or DMSO control during differentiation from naive T cells. (C) Real-time RT-PCR analysis of Foxp3 mRNA levels in iTregs described in (B). Data in (B) and (C) are presented as fold induction by Ex-527 treatment relative to DMSO vehicle (100%). The averages (mean ± SD) of three independent experiments are shown.