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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: J Immunol. 2012 Feb 6;188(6):2712–2721. doi: 10.4049/jimmunol.1100903

Figure 7. Ex-527 promotes the stability of iTregs in vivo and nTregs ex vivo.

Figure 7

(A) Flow cytometric analysis of CD4 and Foxp3 (% in black and MFI in gray) in Tregs isolated from the draining lymph nodes of Boy/J mice 2 weeks after adoptive transfer. Highly sorted iTregs differentiated in vitro from naive CD4+ T cells were transferred into Boy/J mice by retro-orbital injection. DMSO and Ex-527 were injected intraperitoneally into these mice every day for 2 weeks. The data shows one representative experiment of three independent experiments with similar results. (B) The left panel shows flow cytometric analysis of GFP in nTregs isolated from GFP-Foxp3 knock-in mice after ex vivo expansion with or without DMSO. The right panel shows western blot analysis of Foxp3 in cell lysates from GFP- and GFP+ cultures before and after expansion. We show one representative experiment of two independent experiments with similar results. (C) Model of how inhibition of SIRT1 deacetylase activity by Ex-527 increases the acetylation at three newly identified lysine residues in Foxp3 and promotes Treg differentiation and stability. See text for details.